منابع مشابه
Factors Involved in the Use of Organic Solvents as Precipitating and Drying Agents of Immune Sera
1. In concentrations of 70 to 75 per cent the organic solvents methyl, ethyl, and propyl alcohols, and acetone cause complete precipitation of serum proteins and produce maximum loss in solubility. We have referred to this concentration range as the critical concentration. 2. As the concentration of the solvents is increased from about 75 per cent precipitation continues complete but loss in so...
متن کاملPurification of TR-b, a Reiter treponeme protein antigen precipitating with antibodies in human syphilitic sera.
TR-b is a Reiter treponeme antigen, cross-reacting with an antigen in Treponema pallidum (Nichols pathogenic strain). Sera from patients with secondary syphilis contain precipitating antibodies against TR-b. The isolation of TR-b from a bacterial sonic extract is described here. It involved five fractionation steps: anion-exchange chromatography (DE-52 Whatman), gel filtration (Ac-A-22 Ultrogel...
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ژورنال
عنوان ژورنال: Journal of Comparative Pathology and Therapeutics
سال: 1903
ISSN: 0368-1742
DOI: 10.1016/s0368-1742(03)80005-x